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anti cd68 primary antibody  (Proteintech)


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    Structured Review

    Proteintech anti cd68 primary antibody
    Anti Cd68 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd68+primary+antibody/APC+Anti-human+CD68/10__1002_slash_fsn3__71700-59-1-8
    Average 96 stars, based on 43 article reviews
    anti cd68 primary antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: The Activation of the Microglial NLRP3 Inflammasome Is Involved in Tuberous Sclerosis Complex-Related Neuroinflammation.
    Article Snippet: .. The cells were subsequently treated with reagents containing 0.5% Triton X-100 and 5% fetal bovine serum for 30 min. Anti-AIF1 primary antibody (recombinant rabbit monoclonal, 1:500; HUABIO, Hangzhou, China) and anti-CD68 primary antibody (mouse monoclonal, 1:500; Proteintech, Wuhan, China) were incubated at 4 ◦C overnight. .. The next day, the residual primary antibody was removed by washing with PBS, and the cells were incubated for 2 h at room temperature with a goat anti-rabbit fluorescent secondary antibody (1:2500, Abcam, Waltham, MA, USA) or a goat anti-mouse fluorescent secondary antibody (1:1000, Abcam, Waltham, MA, USA).

    Article Title: The Activation of the Microglial NLRP3 Inflammasome Is Involved in Tuberous Sclerosis Complex-Related Neuroinflammation
    Article Snippet: .. The cells were subsequently treated with reagents containing 0.5% Triton X-100 and 5% fetal bovine serum for 30 min. Anti-AIF1 primary antibody (recombinant rabbit monoclonal, 1:500; HUABIO, Hangzhou, China) and anti-CD68 primary antibody (mouse monoclonal, 1:500; Proteintech, Wuhan, China) were incubated at 4 °C overnight. .. The next day, the residual primary antibody was removed by washing with PBS, and the cells were incubated for 2 h at room temperature with a goat anti-rabbit fluorescent secondary antibody (1:2500, Abcam, Waltham, MA, USA) or a goat anti-mouse fluorescent secondary antibody (1:1000, Abcam, Waltham, MA, USA).

    Incubation:

    Article Title: The Activation of the Microglial NLRP3 Inflammasome Is Involved in Tuberous Sclerosis Complex-Related Neuroinflammation.
    Article Snippet: .. The cells were subsequently treated with reagents containing 0.5% Triton X-100 and 5% fetal bovine serum for 30 min. Anti-AIF1 primary antibody (recombinant rabbit monoclonal, 1:500; HUABIO, Hangzhou, China) and anti-CD68 primary antibody (mouse monoclonal, 1:500; Proteintech, Wuhan, China) were incubated at 4 ◦C overnight. .. The next day, the residual primary antibody was removed by washing with PBS, and the cells were incubated for 2 h at room temperature with a goat anti-rabbit fluorescent secondary antibody (1:2500, Abcam, Waltham, MA, USA) or a goat anti-mouse fluorescent secondary antibody (1:1000, Abcam, Waltham, MA, USA).

    Article Title: The Activation of the Microglial NLRP3 Inflammasome Is Involved in Tuberous Sclerosis Complex-Related Neuroinflammation
    Article Snippet: .. The cells were subsequently treated with reagents containing 0.5% Triton X-100 and 5% fetal bovine serum for 30 min. Anti-AIF1 primary antibody (recombinant rabbit monoclonal, 1:500; HUABIO, Hangzhou, China) and anti-CD68 primary antibody (mouse monoclonal, 1:500; Proteintech, Wuhan, China) were incubated at 4 °C overnight. .. The next day, the residual primary antibody was removed by washing with PBS, and the cells were incubated for 2 h at room temperature with a goat anti-rabbit fluorescent secondary antibody (1:2500, Abcam, Waltham, MA, USA) or a goat anti-mouse fluorescent secondary antibody (1:1000, Abcam, Waltham, MA, USA).

    Staining:

    Article Title: Low‐intensity pulsed ultrasound attenuates cardiac inflammation of CVB 3‐induced viral myocarditis via regulation of caveolin‐1 and MAPK pathways
    Article Snippet: .. The sections were then stained with anti‐CD68 primary antibody [dilution 1:500; Proteintech, 25747‐1‐AP] at 4°C overnight and FITC‐conjugated goat anti‐rabbit IgG [dilution 1:500; Yeasen, 33107ES60] at room temperature for 1 hour to identify monocytes/macrophages. .. The fluorescent dye 4′,6‐diamidino‐2‐phenylindole dihydrochloride (DAPI) (beyotime, C1005) was finally added to the sections to mark the nuclei.

    Article Title: Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways.
    Article Snippet: .. The sections were then stained with anti‐CD68 primary antibody [dilution 1:500; Proteintech, 25747‐1‐AP] at 4°C overnight and FITC‐conjugated goat anti‐ rabbit IgG [dilution 1:500; Yeasen, 33107ES60] at room temperature ZHENG ET AL. | 1965 15824934, 2019, 3, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .14098 by IN A SP - N E PA L , W iley O nline L ibrary on [09/01/2025]. ..



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    A HE: It can be seen that the cardiomyocytes in the model group are significantly enlarged and the nuclei become sparse. Sirius red staining: Some areas of myocardium in the model group showed an increase in cell spacing accompanied by fibrotic tissue metaplasia. In contrast, myocytes in the control group were neatly arranged and densely distributed, with significantly less fibrous tissue than in the model group. “LV”: left ventricle. “RV”: right ventricle. B In the model group, a small number of apoptotic cells were observed with green fluorescence, but no large aggregates were detected. C Additionally, there were numerous <t>CD68-positive</t> cells expressing red fluorescence, which were irregularly distributed on the cell wall and in the cytoplasm
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    Image Search Results


    A HE: It can be seen that the cardiomyocytes in the model group are significantly enlarged and the nuclei become sparse. Sirius red staining: Some areas of myocardium in the model group showed an increase in cell spacing accompanied by fibrotic tissue metaplasia. In contrast, myocytes in the control group were neatly arranged and densely distributed, with significantly less fibrous tissue than in the model group. “LV”: left ventricle. “RV”: right ventricle. B In the model group, a small number of apoptotic cells were observed with green fluorescence, but no large aggregates were detected. C Additionally, there were numerous CD68-positive cells expressing red fluorescence, which were irregularly distributed on the cell wall and in the cytoplasm

    Journal: Journal of Cardiovascular Translational Research

    Article Title: Extension of Atherosclerosis ApoE-/- Mouse—a Model of Chronic Myocardial Ischemia and Evaluation Method

    doi: 10.1007/s12265-025-10734-8

    Figure Lengend Snippet: A HE: It can be seen that the cardiomyocytes in the model group are significantly enlarged and the nuclei become sparse. Sirius red staining: Some areas of myocardium in the model group showed an increase in cell spacing accompanied by fibrotic tissue metaplasia. In contrast, myocytes in the control group were neatly arranged and densely distributed, with significantly less fibrous tissue than in the model group. “LV”: left ventricle. “RV”: right ventricle. B In the model group, a small number of apoptotic cells were observed with green fluorescence, but no large aggregates were detected. C Additionally, there were numerous CD68-positive cells expressing red fluorescence, which were irregularly distributed on the cell wall and in the cytoplasm

    Article Snippet: Tissue sections underwent blocking with 5% bovine serum albumin (BSA) (Sigma-Aldrich Cat# A7906) in PBST (0.1% Tween-20 in PBS) for 1 h at room temperature, followed by overnight incubation at 4 °C with anti-CD68 primary antibody (Rabbit polyclonal, Bioss Antibodies, Cat# bs-20403R, RRID: AB_10855800) diluted 1:100 in blocking solution.

    Techniques: Staining, Control, Fluorescence, Expressing